Fig. 5

High expression of FOXO1 in neutrophils increases myelin damage. a Proteomic analysis of brain tissues from mice. The top 30 proteins are enriched terms by GO analysis (group of TBI with depression vs. group of TBI without depression in WT mice). b Immunostaining of MBP (green) and nuclei (DAPI, blue) in the brain tissue of different groups (n = 5). Scale bar = 50 μm. c Electron microscopy image of corpus callosum sections and quantification of myelinated axon number and g-ratio (n = 6). Scale bar = 1 μm. d Western blotting showing the expression of MBP in neutrophils in the TBI mouse model (n = 3). In each group, three biologically independent samples were represented. Data are represented as the mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns non-significant. FOXO1 forkhead box protein O1, GO Gene Ontology, TBI traumatic brain injury, MBP myelin basic protein, DAPI 4,6-diamidino-2-phenylindole dihydrochloride